Social Links

Follow on Facebook Follow on Twitter Follow on G+Follow EiR on PinterestFollow EiR on Instagram

Xpert Access

×

Login To Get Involved!


Forgot your username?


Forgot your password?

DNRS Roof Banner

 

DNRS Interactive DVD Series & Seminars

Modulation of cytokine gene expression in spleen and Peyers patches

 

 

 

J Dig Dis. 2009 Feb;10(1):49-54.

 

Modulation of cytokine gene expression in spleen and Peyer's patches by feeding dahi containing probiotic Lactobacillus casei in mice.

 

Jain S, Yadav H, Sinha PR, Marotta F. Animal Biochemistry Division, National Diary Research Institute, Haryana, India. This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

OBJECTIVE: In present study, feeding effect of probiotic dahi containing Lactobacillus casei on immune system in terms of cytokine gene expression in the spleen and Peyer's patches of mice was evaluated.

 

METHODS: Animals were divided into three groups and fed with; synthetic diet [control group (CD)], dahi containing mixed dahi culture [control dahi-fed group (CDF)]; and probiotic dahi fed group (PDF) for 28 days. The mRNA levels of IL-2, IL-4, IL-6 and IFN-gamma were examined after 14 and 28 days. Total lactobacilli and lactococci counts were determined in the feces.

 

RESULTS: The mRNA levels of IFN-gamma in both spleen and Peyer's patches was found to be significantly increased in PDF animals after 14 and 28 days (P < 0.05) compared with CD and CDF groups. The abundance of IL-2 mRNA also increased significantly in the Peyer's patches of PDF-fed animals. No significant changes were observed in mRNA levels of IL-4 and IL-6 in both spleen and Peyer's patches during whole experimental period. Further, total fecal lactobacilli and lactococci counts in the PDF group were significantly increased during first 10 days, then remained higher up to day 28 compared to other two groups.

 

CONCLUSION: It is concluded that feeding probiotic dahi enhanced the expression of Th1 type cytokines (IFN-gamma and IL-2), especially in the mucosal immune organ (Peyer's patches) rather than in systemic organs (the spleen). This indicates that feeding with probiotic dahi may strengthen the host immune system and protect against the progression of various immune-mediated diseases.

 

PMID: 19236547 [PubMed - indexed for MEDLINE]

 


 

 

Related Articles:

 

  • No comments found

Leave your comments

Post comment as a guest

0 Character restriction
Your text should be more than 25 characters
Your comments are subjected to administrator's moderation.
terms and condition.

Adsense Responsive BottomBanner