Gut. 2006 Jun 15; [Epub ahead of print]
Zinc carnosine, a health food supplement that stabilises small bowel integrity and stimulates gut repair processes.
Mahmood A, Fitzgerald AJ, Marchbank T, Ntatsaki E, Murray D, Ghosh S, Playford RJ.
Dept Gastroenterology, Imperial College, London, United Kingdom.
BACKGROUND: Zinc carnosine (ZnC), is a health food product claimed to possess health-promoting and gastrointestinal supportive activity. Scientific evidence underlying these claims is, however, limited. We, therefore, examined the effect of ZnC on various models of gut injury and repair and in a clinical trial. METHODS: In vitro studies utilised pro-migratory (wounded monolayer) and proliferation ([3H] thymidine incorporation) assays of human colonic (HT29), rat intestinal (RIE) and canine kidney (MDCK) epithelial cells. In vivo studies utilised rat gastric (indomethacin/restraint) and mouse small intestinal (indomethacin) damage models. Healthy volunteers (n=10) undertook a randomised cross-over trial comparing changes in gut permeability (lactulose/rhamnose ratios, L/R) before and after 5 days of indomethacin (50 mg tds, po.) with ZnC (37.5 mg bd) or placebo co-administration. RESULTS: ZnC stimulated migration and proliferation in a dose-dependent manner (maximum effects in both assays at 100microM using HT29 cells), causing an approximate three fold increase in migration or proliferation (both p < 0.01). Oral ZnC decreased gastric (75% reduction at 5mg/ml) and small intestinal injury (50% reduction in villus shortening at 40 mg/ml), both p< 0.01. In volunteers, indomethacin caused a three- fold increase in gut permeability in the control arm; L/R ratio 0.35 +/- 0.035 prior to indomethacin and 0.88 +/- 0.11 (mean +/- SEM) after 5 days indomethacin (p < 0.01), whereas no significant increase in permeability was seen when ZnC was co-administered. CONCLUSION: These initial studies suggest that ZnC, at concentrations likely to be found in the gut lumen, stabilises gut mucosa. Further studies appear warranted.
PMID: 16777920 [PubMed - as supplied by publisher]
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